Characterizing a thermostable Cas9 for bacterial genome editing and silencing
11 Pages · 2017 · 1.81 MB · English
Mihris I.S. Naduthodi1, Alex Gussak1, Rudolf B.L. Brinkman2, Richard van Kranenburg 1,2 & John van der Oost1. CRISPR-Cas9-based genome engineering tools have selected for culturing under microaerobic lactate-producing conditions for 24h44. The growth of the pThermoCas9i_ldhL cultures
Therapeutic miR-21 Silencing Ameliorates Diabetic Kidney Disease in Mice
16 Pages · 2016 · 5.03 MB · English
Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, 30625 Hannover, Germany; 7Division of Nephrology, University end-stage renal disease (ESRD) in the Western world, its molecular . miR-21 expression in human kidney biopsies of diabetic patients.
Genome-Wide Analysis of Epigenetic Silencing Identifies BEX1 and BEX2 as Candidate Tumor ...
11 Pages · 2006 · 456 KB · English
The scope of epigenetic gene silencing in malignant glioma has not been well defined. Malignant glioma, the most Eckstein Medical Research Building, Iowa City, IO 52242. Phone: 319-3358491; Fax: 319-. 3358078; E-mail: .. Parkinson's disease. RaLP. +. Angiogenesis, EGFR signaling pathway,
Multi-generational silencing dynamics control cell aging
21 Pages · 2017 · 5.85 MB · English
Cellular aging plays an important role in many diseases 1, such as cancers 2, metabolic syndromes 3 .. To construct the microfluidic device, designs were first drawn in AutoCAD (Autodesk Inc.). Modeling . microfluidic device was taped to a customized device holder inserted onto the motorized stage.
Enteral siRNA delivery technique for therapeutic gene silencing in the liver via the lymphatic route
13 Pages · 2015 · 1.48 MB · English
penetration with an absorbefacient and in vivo fabrication of a drug delivery system (DDS) with an endogenous carrier in the lymphatic; this enabled enteral and hepatocyte-specific siRNA delivery and therapeutic gene silencing, leading to “oral RNAi therapy.” Results. Formulation of Toc-siRNA i
Highly efficient gene silencing using perfect complementary artificial miRNA targeting AP1 or ...
12 Pages · 2009 · 457 KB · English
potent gene silencing agent against AP1, achieving a desired null mutation RNAs, namely short interfering RNAs (siRNAs) and mi- miRNA duplex. The miRNA strand complementary to its target gene is then recognized by the RISC and guides the complex to degrade the target mRNA (Bartel 2004).
Tissue homogeneity requires inhibition of unequal gene silencing during development
18 Pages · 2016 · 3.24 MB · English
the intestinal lineage, the first cell division results in anticor- related expression of repetitive DNA among daughter cells and subsequent cell divisions result in correlated expression of re- petitive DNA among daughter cells (Fig. 6, G–K) despite the spatial separation of lineal sister cells
RNA interference: a potential therapeutic tool for silencing
8 Pages · 2006 · 359 KB · English
INTRODUCTION The majority of metazoan protein-coding genes contain intervening sequences (introns) whose removal from precursor messenger RNAs
Two classes of small antisense RNAs in fungal RNA silencing triggered by nonintegrative transgenes
9 Pages · 2013 · 248 KB · English
genes have been used in N.crassa as visual reporters of gene silencing, since any alteration of the carotenogenic pathway can be easily detected as a colour change in the. Two classes of small antisense RNAs in fungal RNA silencing triggered by non-integrative transgenes. The EMBO Journal Vol.
Histone deacetylases mediate the silencing of miR-15a, miR-16, and miR-29b in chronic ...
12 Pages · 2012 · 713 KB · English
pare miR-29b expression in CLL may afford a superior comparator to the 2 cord blood samples used as the normal control in the other study. HDAC inhibition restored H3K4me2 marks and robustly induced the expression of 17 of 46 (37%) samples, indicating that a significant number of samples were
Charged residues in the H-NS linker drive DNA binding and gene silencing in single cells
6 Pages · 2017 · 1.28 MB · English
Nucleoid-associated proteins (NAPs) facilitate chromosome orga- nization in bacteria, but the precise mechanism remains elusive. H-NS is a NAP that also plays a major role in silencing pathogen genes. We used genetics, single-particle tracking in live cells, superresolu- tion microscopy, atomic for
Implementation of Refractory Foam Technology for Silencing
123 Pages · 2005 · 4.43 MB · English
The final silencer design boasted an A- weighted overall .. Figure 5.9: Average frequency spectrums for the airplane silencer and no silencer cases
Highly Specific Gene Silencing by Artificial miRNAs in Rice
10 Pages · 2008 · 458 KB · English
037900), and by the USAID-funded project entitled Development of rice biotechnology products for Asia: technical and pre-regulatory components at the such as increased yield or biotic and abiotic stress tolerance [1–3]. all genes in important crops such as wheat, barley and maize have.
RNA stem structure governs coupling of dicing and gene silencing in RNA interference
10 Pages · 2017 · 1.47 MB · English
RNAi is a multistep process that initiates from the biogenesis of miRNA or siRNA from precursor RNAs and results in the inhibition of a target gene by an RNA-induced silencing complex. (RISC) (6–8). First, a precursor RNA (premiRNA or presiRNA) is trimmed down to 21- to 23-bp dsRNA (miRNA or