Development of RNA Interference Trigger-Mediated Gene Silencing in Entamoeba invadens
12 Pages · 2016 · 2.15 MB · English
proach to successfully silence two E. invadens genes: a putative rhomboid protease gene and a SHAQKY family Myb gene. The. Myb gene is upregulated April 2016 Volume 84 Number 4. Infection and Immunity . ously described (36) using 100 g of sRNA-enriched fraction. Samples were run on a
HLTF gene silencing in human colon cancer
8 Pages · 2002 · 378 KB · English
This paper was submitted directly (Track II) to the PNAS office. Abbreviations: HLTF, helicase-like transcription factor; RT-PCR, reverse transcription–PCR;. MS-PCR, methylation-specific PCR; MLH1, MutL homolog1; CIMP, CpG island methylator phenotype; 5-azaC, azacytidine. †H.R.M. and W.-D.C.
Chapter 10: Shh: Silencing the Hedgehog Pathway
18 Pages · 2007 · 539 KB · English
When Shh (Sonic hedgehog) ligand binds to Ptch, then Smo is activated, the signal is transduced, and transcription and cell division result.
Recurrent ubiquitin B silencing in gynecological cancers establishes dependence on ubiquitin C
15 Pages · 2017 · 13.44 MB · English
Alexia T. Kedves,1 Scott Gleim,1 Xiaoyou Liang,1 Dennis M. Bonal,2 Frederic Sigoillot,1 Fred Harbinski,1 Sneha Sanghavi,3. Christina Benander genes is recurrent- ly silenced establishes a rationale for exploring the impact of this that were collated and summarized using python v3.4. Determina-.
Gene silencing mechanisms in Phytophthora infestans
68 Pages · 2012 · 7.75 MB · English
Faculty of Natural Resources and Agricultural sciences Additionally, six miRNA candidates, and sRNAs that mapped to mitochondrial, tRNA and genomic . RNAi. RNA interference. rRNA ribosomal RNA. RxLR. Effector protein containing Arginine-any amino acid-Leucine-Arginine motif. siRNA.
Prosthetic systems for therapeutic optical activation and silencing of genetically-targeted neurons
16 Pages · 2008 · 1.66 MB · English
An ideal therapy data, and wrote the paper paper with a demonstration of light-evoked neural activity in a non-human primate. 2. distance ended the trip of the photon packet within the starting voxel, then the packet would.
Understanding Transgene Silencing and Environmental Stress Adaptability in Chlamydomonas ...
74 Pages · 2013 · 2.69 MB · English
growing algae outdoors is battling with environmental stresses, which The 2A sequence was used in conjunction with the Venus-Bcl-xL sequence to a high salt concentration (150mM or 250mM NaCl) or photooxidative stress.
Viral Class 1 RNase III Involved in Suppression of RNA Silencing
12 Pages · 2005 · 1003 KB · English
products can be observed at the bottom of the gel (dsRNA panel, lanes 50, 110, and 130 mM). (E to G) genome of Chilo iridescent virus. Virology
Nickel Ions Increase Histone H3 Lysine 9 Dimethylation and Induce Transgene Silencing
10 Pages · 2006 · 400 KB · English
nickel ions induce transgene silencing by increasing histone H3K9 dimethylation, and this effect is mediated by the inhibition of Among them, methylation of histone H3 lysine 9 (H3K9) is one of the Toxicol. Appl. Pharma- col.
Characterizing a thermostable Cas9 for bacterial genome editing and silencing
11 Pages · 2017 · 1.81 MB · English
Mihris I.S. Naduthodi1, Alex Gussak1, Rudolf B.L. Brinkman2, Richard van Kranenburg 1,2 & John van der Oost1. CRISPR-Cas9-based genome engineering tools have selected for culturing under microaerobic lactate-producing conditions for 24h44. The growth of the pThermoCas9i_ldhL cultures
Therapeutic miR-21 Silencing Ameliorates Diabetic Kidney Disease in Mice
16 Pages · 2016 · 5.03 MB · English
Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, 30625 Hannover, Germany; 7Division of Nephrology, University end-stage renal disease (ESRD) in the Western world, its molecular . miR-21 expression in human kidney biopsies of diabetic patients.
Genome-Wide Analysis of Epigenetic Silencing Identifies BEX1 and BEX2 as Candidate Tumor ...
11 Pages · 2006 · 456 KB · English
The scope of epigenetic gene silencing in malignant glioma has not been well defined. Malignant glioma, the most Eckstein Medical Research Building, Iowa City, IO 52242. Phone: 319-3358491; Fax: 319-. 3358078; E-mail: .. Parkinson's disease. RaLP. +. Angiogenesis, EGFR signaling pathway,
Multi-generational silencing dynamics control cell aging
21 Pages · 2017 · 5.85 MB · English
Cellular aging plays an important role in many diseases 1, such as cancers 2, metabolic syndromes 3 .. To construct the microfluidic device, designs were first drawn in AutoCAD (Autodesk Inc.). Modeling . microfluidic device was taped to a customized device holder inserted onto the motorized stage.
Enteral siRNA delivery technique for therapeutic gene silencing in the liver via the lymphatic route
13 Pages · 2015 · 1.48 MB · English
penetration with an absorbefacient and in vivo fabrication of a drug delivery system (DDS) with an endogenous carrier in the lymphatic; this enabled enteral and hepatocyte-specific siRNA delivery and therapeutic gene silencing, leading to “oral RNAi therapy.” Results. Formulation of Toc-siRNA i
Highly efficient gene silencing using perfect complementary artificial miRNA targeting AP1 or ...
12 Pages · 2009 · 457 KB · English
potent gene silencing agent against AP1, achieving a desired null mutation RNAs, namely short interfering RNAs (siRNAs) and mi- miRNA duplex. The miRNA strand complementary to its target gene is then recognized by the RISC and guides the complex to degrade the target mRNA (Bartel 2004).