Proximate Analysis, Phytochemical Screening and Antimicrobial

Proximate Analysis, Phytochemical Screening and Antimicrobial

6 Pages · 2015 · 335 KB · English

The aqueous extract of baobab leaves was tested for its antimicrobial activity against 10 µg/ml(Bacterial), tioconazole70% (fungi) as negative and positive controls respectively. numerous other wild – gathered foods, and are a rich source of calcium(Glew et al, 1997; Barminas et al, 1998;.

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IOSR Journal of Applied Chemistry (IOSR JAC) eISSN: 2278 5736Volume 8, Issue 5 Ver I (May 2015), PP 60 65 wwwiosrjournalsorg DOI: 109790/5736 0851 60 65 wwwiosrjournalsorg 60 |Page Proximate Analysis, Phytochemical Screening and Antimicrobial Activity of Baobab (Adansonia digitata ) Leaves 1Abiona DL, 2Adedapo Z 3Suleiman MK 1,2,3 Department of Chemistry, The Polytechnic, Ibadan, Oyo State, Nigeria Abstract: Baobab (Adansonia digitata L) is known in Africa as the tree of life because there are many traditional uses for every part of it, from the leaves to the roots This study carried out the proximate analysis of baobab leaves, screened the phytochemicals present and determined the leaves’ aqueous extract antimicrobial activity to suit the traditional way of preparation and use Phytochemicals screening was carried out on the ethanolic , methanolic and aqueous extracts of the leave s The phytochemicals screened were reducing sugar, flavonoids, terpenoids, saponins, tannins, alkaloids, anthraquinones, steroids, resins, phenols, cardiac active glycosides The aqueous extract of baobab leav es was tested for its antimicrobial activity against the following organisms; Staphylococcus aureus, Esherichia coli , Bacillus subtilis , Pseudomonas aeruginosa, Saimonelle typhi , Klebsiellae pneumonae , Candida albicans , Aspergillus niger , Rhizopus stolomifer , Penicillum rotatum at at different concentrations of 200, 100, 50, 25, 125, 625 mg/ml, with water, gentamycin 10 µg/ml(Bacterial), tioconazole70% (fungi) as negative and positive controls respectively The proximate analysis revealed baobab leaves contain protein (136%), fat (271`%), sugar(001%), ash(408%), crude fibre (245%), Moisture content(782%),Vitamin C (1498mg/100g) All phytochemicals screened were found to be present The result showed that the aqueous extract of the baobab leaves was active against the tested organisms except klebsiellae pneumonae Staphylococcus aureus is more sensitive to the antimicrobial activity of the extract The aqueous extract displayed broad spectrum effect because it has activities on Grams positi ve and Grams negative bacteria and fungi Keywords: Adansonia digitata, Staphylococcus aureus , Esherichia coli , Bacillus subtilis , Pseudomonas aeruginosa, Saimonelle typhi , Klebsiellae pneumonae , Candida albicans , Aspergillus niger , Rhizopus stolomifer , Penicillum rotatum I Introduction Baobab ( A digitata ), a tree plant belonging to the Malvaceae family, is widespread throughout the hot, drier regions of tropical Africa (De Caluwé et al, 2010; Bremer et al , 2003;FAO,

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